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Proceedings Paper

Liposome characterization with fluorescence cumulant analysis
Author(s): Joseph E. Reiner; Andreas Jahn; Laurie E. Locascio; Michael Gaitan; John J. Kasianowicz
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Paper Abstract

Liposomes are self-assembled spherical vesicles comprised of a lipid bilayer membrane that segregates an internal aqueous environment from an external aqueous environment. These nanometer-scale structures have demonstrated potential for targeted drug delivery applications. For liposomes to be useful in vivo, the liposome size and dosage of molecules contained within them needs to be controlled. We present here a fluorescence-based technique for characterizing the relative encapsulation efficiency, leakage rate, and shelf life of liposome formulations. We report results from three different liposome solutions over a period of two months that show the liposome brightness remains stable while the background dye concentration increases. These parameters may prove useful for optimizing the liposome formation process.

Paper Details

Date Published: 8 June 2007
PDF: 11 pages
Proc. SPIE 6602, Noise and Fluctuations in Biological, Biophysical, and Biomedical Systems, 66020I (8 June 2007); doi: 10.1117/12.726803
Show Author Affiliations
Joseph E. Reiner, National Institute of Standards and Technology (United States)
Andreas Jahn, National Institute of Standards and Technology (United States)
Laurie E. Locascio, National Institute of Standards and Technology (United States)
Michael Gaitan, National Institute of Standards and Technology (United States)
John J. Kasianowicz, National Institute of Standards and Technology (United States)


Published in SPIE Proceedings Vol. 6602:
Noise and Fluctuations in Biological, Biophysical, and Biomedical Systems
Sergey M. Bezrukov, Editor(s)

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