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Proceedings Paper

Laser fluorescence fluctuation excesses in molecular immunology experiments
Author(s): N. E. Galich; M. V. Filatov
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Paper Abstract

A novel approach to statistical analysis of flow cytometry fluorescence data have been developed and applied for population analysis of blood neutrophils stained with hydroethidine during respiratory burst reaction. The staining based on intracellular oxidation hydroethidine to ethidium bromide, which intercalate into cell DNA. Fluorescence of the resultant product serves as a measure of the neutrophil ability to generate superoxide radicals after induction respiratory burst reaction by phorbol myristate acetate (PMA). It was demonstrated that polymorphonuclear leukocytes of persons with inflammatory diseases showed a considerably changed response. Cytofluorometric histograms obtained have unique information about condition of neutrophil population what might to allow a determination of the pathology processes type connecting with such inflammation. A novel approach to histogram analysis is based on analysis of high-momentum dynamic of distribution. The features of fluctuation excesses of distribution have unique information about disease under consideration.

Paper Details

Date Published: 10 April 2007
PDF: 4 pages
Proc. SPIE 6597, Nanodesign, Technology, and Computer Simulations, 65970L (10 April 2007); doi: 10.1117/12.726756
Show Author Affiliations
N. E. Galich, St. Petersburg Polytechnical Univ. (Russia)
M. V. Filatov, B.P. Konstantinov Petersburg Nuclear Physics Institute (Russia)

Published in SPIE Proceedings Vol. 6597:
Nanodesign, Technology, and Computer Simulations
Alexander I. Melker; Teodor Breczko, Editor(s)

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