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Proceedings Paper

Simultaneous imaging of confocal fluorescence and raman spectrum
Author(s): MyoungKi Ahn; Taehoon Kim; HongKi Yoo; InCheon Song; DaeGab Gweon
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Paper Abstract

The advantage of confocal fluorescence microscopy is the ability to acquire high resolution images of fluorescent specimens non-invasively. On the other hand, the advantage of Raman microscopy is the ability to provide the chemical characteristics of specimens from spectroscopy. To obtain simultaneously high resolution images and chemical characteristics of specimens, fluorescence signals from stained cell and Raman spectrum from cell itself should be separated. By separating two kinds of signals, confocal fluorescence image and Raman spectrum are acquired simultaneously at the same position. In this paper, we demonstrate a confocal fluorescence microscopy combined with the Raman microscopy. And we propose a method that eliminates Raman spectrum of fluorophore itself from Raman spectrum of the stained cell and that obtains simultaneously confocal fluorescence image from stained cell and Raman spectrum of cell itself without replacing a cell.

Paper Details

Date Published: 23 July 2007
PDF: 9 pages
Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 663011 (23 July 2007); doi: 10.1117/12.726688
Show Author Affiliations
MyoungKi Ahn, Korea Advanced Institute of Science and Technology (South Korea)
Taehoon Kim, Korea Advanced Institute of Science and Technology (South Korea)
HongKi Yoo, Korea Advanced Institute of Science and Technology (South Korea)
InCheon Song, Korea Advanced Institute of Science and Technology (South Korea)
DaeGab Gweon, Korea Advanced Institute of Science and Technology (South Korea)


Published in SPIE Proceedings Vol. 6630:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
Tony Wilson; Ammasi Periasamy, Editor(s)

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