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Lateral shear and digital holographic microscopy to check dynamic behaviour of biological cellFormat | Member Price | Non-Member Price |
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Paper Abstract
Quantitative Phase Microscopy (QPM) by means of Lateral Shear Interferometry (LSI) is presented as a metrological
tool to investigate dynamical behaviour of living cells. QPM achieved by a Digital Holographic Microscope (DHM), in
transmission geometry, allows to reconstruct object shape from the numerical reconstruction of the complex field
transmitted by the object. This geometry is useful in special case of biological material because they present an
amplitude contrast too small for quantitative amplitude microscopy. DHM realized with a Mach-Zehnder interferometer
provides the object phase map which is simply related to the object height. This technique gives a detailed map of the
internal structure of the cell. The main trouble in QPM is the removal of the aberration due to the optical setup. Usually,
in DHM the aberrations are eliminated by subtraction of a reference phase map (acquired without sample cell) from the
object phase map. We propose a method, based on LSI, for the aberration removal that avoids this double exposure and
makes use of the single object hologram. The reconstructed wavefront and its shifted replica are subtracted to obtain a
shearogram from which the phase map of the object can be completely retrieved. Both shifted replica and subtraction
are processed numerically. This method allows to remove optical aberrations more efficiently and faster than other
conventional techniques and provides real time monitoring of cell samples.
Paper Details
Date Published: 18 June 2007
PDF: 6 pages
Proc. SPIE 6617, Modeling Aspects in Optical Metrology, 661706 (18 June 2007); doi: 10.1117/12.726153
Published in SPIE Proceedings Vol. 6617:
Modeling Aspects in Optical Metrology
Harald Bosse; Bernd Bodermann; Richard M. Silver, Editor(s)
PDF: 6 pages
Proc. SPIE 6617, Modeling Aspects in Optical Metrology, 661706 (18 June 2007); doi: 10.1117/12.726153
Show Author Affiliations
Lisa Miccio, Istituto Nazionale di Ottica Applicata del CNR (Italy)
LENS (Italy)
Simonetta Grilli, Istituto Nazionale di Ottica Applicata del CNR (Italy)
Sergio De Nicola, Istituto di Cibernetica E. Caianiello del CNR (Italy)
LENS (Italy)
Simonetta Grilli, Istituto Nazionale di Ottica Applicata del CNR (Italy)
Sergio De Nicola, Istituto di Cibernetica E. Caianiello del CNR (Italy)
Andrea Finizio, Istituto di Cibernetica E. Caianiello del CNR (Italy)
Pietro Ferraro, Istituto Nazionale di Ottica Applicata del CNR (Italy)
Pietro Ferraro, Istituto Nazionale di Ottica Applicata del CNR (Italy)
Published in SPIE Proceedings Vol. 6617:
Modeling Aspects in Optical Metrology
Harald Bosse; Bernd Bodermann; Richard M. Silver, Editor(s)
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