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Proceedings Paper

Research and fabrication of integrated bio-sensor for blood analysis based on uTAS
Author(s): De En; Jianxia Wei; Zhengrong Tong; Caihe Chen; Yuming Cui; Kexin Xu; Qin Si; Chao Li; Jie Liu
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Paper Abstract

For simultaneously detecting multi-parameters of blood in the clinical diagnosis, the analysis apparatus should be smaller in size, more reliable and sensitive. So a kind of integrated bio-sensor for blood analysis based on Micro Total Analysis System (μTAS) is presented. It provides modern bio-sensor prospect with a novel technology. A multi-parameters of blood analysis integration sensor is μTAS bio-sensor based on 4 groups of interdigital array (IDA)microelectrodes. The IDA microelectrodes are fabricated on glass substrates by photography, film deposition and other microfabrication techniques. Thin-film gold microelectrode with a thickness of 250nm is deposited on a chromium-adhesion layer. The finger microelectrode width and space are both 10μm. The work space is 2×2cm2. The concentration of Blood sugar, Total Cholesterol, Acetone body and Lactic acid is measured by detecting steady-state limiting currents in IDA microelectrodes modified with enzymes on the "generate-collect" mode. Blood distribution structure is designed and fabricated, to distribute blood and isolate reaction areas. By contrasting two kinds of process flow based on lift-off and etching, etching is adopted to preparation method of microelectrode. A multi-channel apparatus for current measurement is accompleted. The system characteristics of the bio-sensor are tested. The curve of the apparatus time to current response is achieved by testing on real-time. The relationships between parameter concentration and current are analyzed in detail. The experimental data indicates: current measurement dimension 0~40μA, certainty of measurement 0.1μA, the performances of the bio-sensor meets design requirement.

Paper Details

Date Published: 29 January 2007
PDF: 6 pages
Proc. SPIE 6279, 27th International Congress on High-Speed Photography and Photonics, 62795Y (29 January 2007); doi: 10.1117/12.725485
Show Author Affiliations
De En, Tianjin Univ. (China)
Inner Mongolia Univ. of Nationalities (China)
Jianxia Wei, Tianjin Navigation Instrument Research Institute (China)
Zhengrong Tong, Tianjin Univ. (China)
Caihe Chen, Tianjin Univ. (China)
Yuming Cui, Tianjin Univ. (China)
Kexin Xu, Tianjin Univ. (China)
Qin Si, Inner Mongolia Univ. of Nationalities (China)
Chao Li, Tianjin Univ. (China)
Jie Liu, Tianjin Zhongke Haixun Co., Ltd. (China)

Published in SPIE Proceedings Vol. 6279:
27th International Congress on High-Speed Photography and Photonics

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