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Proceedings Paper

Ligand-bound quantum dots for intracellular imaging of neural receptors
Author(s): Tania Q. Vu; Sujata Sundara Rajan; Hongyan Liu
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Paper Abstract

Quantum dots (QDs) may serve as improved platforms for the complex modulation and ultra-sensitive imaging of molecular signaling in cells. The time course and spatial localization of activated ligand-receptor complexes and their trafficking within cells is becoming increasingly understood as vital for propagating cell signals. However, the movement and fate of ligand-receptor pairs inside cells is difficult to define with current technologies. We have studied the intracellular trafficking of TrkA receptors using QDs conjugated with nerve growth factor, a neuropeptide ligand critical for nervous system development and regulation. We find that NGF-QDs bind and activate TrkA surface receptors in PC12 neurons. Spatiotemporal maps of TrkA-NGF-QD endocytosis and translocation can be directly visualized with single QD resolution. Moreover, single molecule tracking experiments indicates that QDs complexes are actively shuttled over long distances within newly-sprouted neuronal processes. These results indicate that QDs can serve as effective high-resolution probe to track ligand-receptor function in the interior of cells.

Paper Details

Date Published: 27 February 2007
PDF: 10 pages
Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 644813 (27 February 2007); doi: 10.1117/12.724220
Show Author Affiliations
Tania Q. Vu, Oregon Health & Science Univ. (United States)
Sujata Sundara Rajan, Oregon Health & Science Univ. (United States)
Hongyan Liu, Oregon Health & Science Univ. (United States)

Published in SPIE Proceedings Vol. 6448:
Colloidal Quantum Dots for Biomedical Applications II
Marek Osinski; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

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