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Proceedings Paper

Analytical applications of aptamers
Author(s): S. Tombelli; M. Minunni; M. Mascini
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Paper Abstract

Aptamers are single stranded DNA or RNA ligands which can be selected for different targets starting from a library of molecules containing randomly created sequences. Aptamers have been selected to bind very different targets, from proteins to small organic dyes. Aptamers are proposed as alternatives to antibodies as biorecognition elements in analytical devices with ever increasing frequency. This in order to satisfy the demand for quick, cheap, simple and highly reproducible analytical devices, especially for protein detection in the medical field or for the detection of smaller molecules in environmental and food analysis. In our recent experience, DNA and RNA aptamers, specific for three different proteins (Tat, IgE and thrombin), have been exploited as bio-recognition elements to develop specific biosensors (aptasensors). These recognition elements have been coupled to piezoelectric quartz crystals and surface plasmon resonance (SPR) devices as transducers where the aptamers have been immobilized on the gold surface of the crystals electrodes or on SPR chips, respectively.

Paper Details

Date Published: 16 May 2007
PDF: 9 pages
Proc. SPIE 6585, Optical Sensing Technology and Applications, 65850W (16 May 2007); doi: 10.1117/12.724172
Show Author Affiliations
S. Tombelli, Univ. degli Studi di Firenze (Italy)
M. Minunni, Univ. degli Studi di Firenze (Italy)
M. Mascini, Univ. degli Studi di Firenze (Italy)

Published in SPIE Proceedings Vol. 6585:
Optical Sensing Technology and Applications
Francesco Baldini; Jiri Homola; Robert A. Lieberman; Miroslav Miler, Editor(s)

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