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Proceedings Paper

Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy
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Paper Abstract

In this work, we demonstrate the use of Two-Photon Fluorescence Lifetime Imaging Microscopy (TP-FLIM) for intracellular calcium ([Ca2+]i) measurement with a calcium sensitive fluorescent dye in a neuronal cell model and brain tissue. Calcium perturbations were induced via chemical stimulation and our results signify the potential of TP-FLIM for quantitative ([Ca2+]i) measurement within the physiological range of intracellular [Ca2+].

Paper Details

Date Published: 23 March 2007
PDF: 4 pages
Proc. SPIE 6424, Photonic Therapeutics and Diagnostics III, 64242L (23 March 2007); doi: 10.1117/12.723959
Show Author Affiliations
Eddy Kuwana, Ctr. for Biomedical Engineering (United States)
Gracie Vargas-Adams, Ctr. for Biomedical Engineering (United States)
Todd C. Pappas, Ctr. for Biomedical Engineering (United States)
Anton V. Liopo, Ctr. for Biomedical Engineering (United States)
Massoud Motamedi, Ctr. for Biomedical Engineering (United States)

Published in SPIE Proceedings Vol. 6424:
Photonic Therapeutics and Diagnostics III
Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Reza S. Malek M.D.; Kenton W. Gregory M.D.; Nikiforos Kollias M.D.; Bernard Choi; Steen J. Madsen; Guillermo J. Tearney M.D.; Justus F. R. Ilgner M.D.; Haishan Zeng, Editor(s)

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