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Photonics and microarray technology
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Paper Abstract

Photonic induced immobilization of biosensor molecules is a novel technology that results in spatially oriented and spatially localized covalent coupling of a large variety of biomolecules onto thiol reactive surfaces, e.g. thiolated glass, quartz, gold or silicon. The reaction mechanism behind the reported new technology involves light-induced breakage of disulphide bridges in proteins upon UV illumination of nearby aromatic amino acids resulting in the formation of reactive molecules that will form covalent bonds with thiol reactive surfaces. This new technology has the potential of replacing present micro dispensing arraying technologies, where the size of the individual sensor spots are limited by the size of the dispensed droplets. Using light-induced immobilization the spatial resolution is defined by the area of the sensor surface that is illuminated by UV light and not by the physical size of the dispensed droplets of sensor molecules. This new technology allows for dense packing of different biomolecules on a surface, allowing the creation of multi-potent functionalized materials, such as biosensors with micrometer sized individual sensor spots. Thus, we have developed the necessary technology for preparing large protein arrays of enzymes and fragments of antibodies, with micrometer resolution, without the need for liquid micro dispensing.

Paper Details

Date Published: 16 May 2007
PDF: 8 pages
Proc. SPIE 6585, Optical Sensing Technology and Applications, 658516 (16 May 2007); doi: 10.1117/12.722927
Show Author Affiliations
E. Skovsen, Univ. of Aalborg (Denmark)
BioNanoPhotonics A/S (Denmark)
M. Duroux, Univ. of Aalborg (Denmark)
BioNanoPhotonics A/S (Denmark)
M. T. Neves-Petersen, Univ. of Aalborg (Denmark)
BioNanoPhotonics A/S (Denmark)
L. Duroux, Univ. of Aalborg (Denmark)
BioNanoPhotonics A/S (Denmark)
S. B. Petersen, Univ. of Aalborg (Denmark)
BioNanoPhotonics A/S (Denmark)

Published in SPIE Proceedings Vol. 6585:
Optical Sensing Technology and Applications
Francesco Baldini; Jiri Homola; Robert A. Lieberman; Miroslav Miler, Editor(s)

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