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Proceedings Paper

Optical principle of pH measurement for detection of auxin flow through cellular membrane
Author(s): Ondrej Podrazky; Jan Mrazek; Miroslav Seidl; Ivan Kasik; Petr Tobiska; Vlastimil Matejec; Tomas Martan; Jan Aubrecht
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Paper Abstract

The paper shows an approach to the determination of pH changes of solutions with a fine spatial resolution by means of fiber-optic tapers and fluorescence detection. This approach can be adopted for the determination of auxin flow through celluar membranes. Spectral absorption and fluorescence of pH transducers, namely of fluorescein, carboxyfluorescein, 6,8-dihydroxy-1,3-pyrenedisulfonic acid disodium salt and 2',7'-bis(2-carbonylethyl)-5(6)-carboxyfluorescein, were tested. The approach, based on the determination of a shift of the maxima of their fluorescence peaks, was employed for processing the measured fluorescence data in bulk solutions. Suitable tapered fiber probes were prepared and in vitro demonstrated for pH monitoring in a pH range from 6 to 7.

Paper Details

Date Published: 16 May 2007
PDF: 7 pages
Proc. SPIE 6585, Optical Sensing Technology and Applications, 65850Y (16 May 2007); doi: 10.1117/12.722924
Show Author Affiliations
Ondrej Podrazky, Institute of Photonics and Electronics ASCR (Czech Republic)
Jan Mrazek, Institute of Photonics and Electronics ASCR (Czech Republic)
Miroslav Seidl, Institute of Photonics and Electronics ASCR (Czech Republic)
Ivan Kasik, Institute of Photonics and Electronics ASCR (Czech Republic)
Petr Tobiska, Institute of Photonics and Electronics ASCR (Czech Republic)
Vlastimil Matejec, Institute of Photonics and Electronics ASCR (Czech Republic)
Tomas Martan, Institute of Photonics and Electronics ASCR (Czech Republic)
Jan Aubrecht, Institute of Photonics and Electronics ASCR (Czech Republic)


Published in SPIE Proceedings Vol. 6585:
Optical Sensing Technology and Applications
Francesco Baldini; Jiri Homola; Robert A. Lieberman; Miroslav Miler, Editor(s)

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