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Proceedings Paper

Nanostructured interfacial architectures for detection of biospecific interactions
Author(s): Praskovia M. Boltovets; Andriy A. Savchenko; Boris A. Snopok
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Paper Abstract

In the present work the interaction between GST (glutathione-S-transferase) and immobilized glutathione on the mixed thiol monolayers was investigated by surface plasmon resonance method. The interfacial structures based on mixed monolayer of CH3-/C(O)OH and OH-/C(O)OH- terminated thiols as an support for the immobilization of glutathione (GSH)where used. The range of matrix/reactive groups ratio was up to 1:700. The standard procedure with activation by NHS/EDC with further applying of PDEA was used for GSH binding. It was demonstrated, that both the nature of the matrix terminal group and the density of the immobilized ligand are important for the efficiency of the protein-surface and protein-protein interaction at the sensor surface. Testing of the surface coupled GST by specific anti-GST antibodies shown the same character of the sensor response versus GSH density.

Paper Details

Date Published: 16 May 2007
PDF: 9 pages
Proc. SPIE 6585, Optical Sensing Technology and Applications, 65851S (16 May 2007); doi: 10.1117/12.722851
Show Author Affiliations
Praskovia M. Boltovets, V. Lashkaryov Institute of Semiconductor Physics (Ukraine)
Andriy A. Savchenko, V. Lashkaryov Institute of Semiconductor Physics (Ukraine)
Boris A. Snopok, V. Lashkaryov Institute of Semiconductor Physics (Ukraine)

Published in SPIE Proceedings Vol. 6585:
Optical Sensing Technology and Applications
Francesco Baldini; Jiri Homola; Robert A. Lieberman; Miroslav Miler, Editor(s)

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