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Proceedings Paper

Design and realization of highly stable porous silicon optical biosensor based on proteins from extremophiles
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Paper Abstract

The interaction between an analyte and a biological recognition system is normally detected in biosensors by the transducer element which converts the molecular event into a measurable effect, such as an electrical or optical signal. Porous silicon microstructures have unique optical and morphological properties that can be exploited in biosensing. The large specific surface area (even greater than 500 m2/cm3) and the resonant optical response allow detecting the effect of a change in refractive index of liquid solutions, which interact with the porous matrix, with very high sensitivity. Moreover, the porous silicon surface can be chemically modified to link the bioprobe which recognize the target analytes, in order to enhance the selectivity and specificity of the sensor device. The molecular probe we used was purified by an extremophile organism, Thermococcus litoralis: the protein is very stable in a wide range of temperatures even if with different behavior respect to the interaction with the ligand.

Paper Details

Date Published: 16 May 2007
PDF: 7 pages
Proc. SPIE 6585, Optical Sensing Technology and Applications, 658517 (16 May 2007); doi: 10.1117/12.722389
Show Author Affiliations
Luca De Stefano, Institute for Microelectronics and Microsystems, CNR (Italy)
Lucia Rotiroti, Institute for Microelectronics and Microsystems, CNR (Italy)
Federico II Univ. of Naples (Italy)
Ilaria Rea, Institute for Microelectronics and Microsystems, CNR (Italy)
Federico II Univ. of Naples (Italy)
Edoardo De Tommasi, Institute for Microelectronics and Microsystems, CNR (Italy)
Annalisa Vitale, Institute of Protein Biochemistry, CNR (Italy)
Mosè Rossi, Institute of Protein Biochemistry, CNR (Italy)
Ivo Rendina, Institute for Microelectronics and Microsystems, CNR (Italy)
Sabato D'Auria, Institute of Protein Biochemistry, CNR (Italy)


Published in SPIE Proceedings Vol. 6585:
Optical Sensing Technology and Applications
Francesco Baldini; Jiri Homola; Robert A. Lieberman; Miroslav Miler, Editor(s)

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