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Proceedings Paper

Noninvasive forward-scattering system for rapid detection, characterization, and identification of bacterial colonies
Author(s): Bartek Rajwa; Bulent Bayraktar; Padmapriya P. Banada; Karleigh Huff; Euiwon Bae; E. Daniel Hirleman; Arun K. Bhunia; J. Paul Robinson
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Paper Abstract

Bacterial contamination of food products puts the public at risk and also generates a substantial cost for the food-processing industry. One of the greatest challenges in the response to these incidents is rapid recognition of the bacterial agents involved. Only a few currently available technologies allow testing to be performed outside of specialized microbiological laboratories. Most current systems are based on the use of expensive PCR or antibody-based techniques, and require complicated sample preparation for reliable results. Herein, we report our efforts to develop a noninvasive optical forward-scattering system for rapid, automated identification of bacterial colonies grown on solid surfaces. The presented system employs computer-vision and pattern-recognition techniques to classify scatter patterns produced by bacterial colonies irradiated with laser light. Application of Zernike and Chebyshev moments, as well as Haralick texture descriptors for image feature extraction, allows for a very high recognition rate. An SVM algorithm was used for classification of patterns. Low error rates determined by cross-validation, reproducibility of the measurements, and robustness of the system prove that the proposed technology can be implemented in automated devices for bacterial detection.

Paper Details

Date Published: 26 April 2007
PDF: 7 pages
Proc. SPIE 6554, Chemical and Biological Sensing VIII, 65540N (26 April 2007); doi: 10.1117/12.722200
Show Author Affiliations
Bartek Rajwa, Purdue Univ. (United States)
Bulent Bayraktar, Purdue Univ. (United States)
Padmapriya P. Banada, Purdue Univ. (United States)
Karleigh Huff, Purdue Univ. (United States)
Euiwon Bae, Purdue Univ. (United States)
E. Daniel Hirleman, Purdue Univ. (United States)
Arun K. Bhunia, Purdue Univ. (United States)
J. Paul Robinson, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 6554:
Chemical and Biological Sensing VIII
Augustus W. Fountain, Editor(s)

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