Share Email Print
cover

Proceedings Paper

Microfluidic device detection of waterborne pathogens through static light scattering of latex immunoagglutination using proximity optical fibers
Author(s): Jeong-Yeol Yoon; Jin-Hee Han; Brian Heinze; Lonnie J. Lucas
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Microfluidic device detections of E. coli K12 in deionized (DI) water and E. coli in field water sample were demonstrated through static light scattering of latex immunoagglutination using proximity optical fibers. This method is a fully-automated, one-step detection, and requires neither sample pre-treatment nor cell culturing often required in many on-chip detections. We have used highly carboxylated polystyrene submicron latex particles without surfactants to enhance diffusional mixing and prevent non-specific bindings towards successful demonstration of latex immunoagglutination in microfluidic device. Detection of E. coli was performed by taking microscopic images from the view cell of a microfluidic device and counting the fractions of non-agglutinated and agglutinated particles. The limit of detection (LOD) was ca. 150 CFU ml-1 with this method for both E. coli K12 in DI water and E. coli in field water sample, indicating no non-specific bindings. Improved LOD of < 4.3 CFU ml-1 was achieved by measuring forward static light scattering from microfluidic device, using proximity optical fibers and a USB-powered miniature spectrometer. The total assay time for sample preparation (mostly dilutions) and on-chip assay (mostly injections and short incubation time) was < 10 min.

Paper Details

Date Published: 3 May 2007
PDF: 7 pages
Proc. SPIE 6556, Micro (MEMS) and Nanotechnologies for Defense and Security, 65560M (3 May 2007); doi: 10.1117/12.721129
Show Author Affiliations
Jeong-Yeol Yoon, The Univ. of Arizona (United States)
Jin-Hee Han, The Univ. of Arizona (United States)
Brian Heinze, The Univ. of Arizona (United States)
Lonnie J. Lucas, The Univ. of Arizona (United States)


Published in SPIE Proceedings Vol. 6556:
Micro (MEMS) and Nanotechnologies for Defense and Security
Thomas George; Zhongyang Cheng, Editor(s)

© SPIE. Terms of Use
Back to Top