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Proceedings Paper

Detection of Bacillus anthracis spores in water using biosensors based on magnetostrictive microcantilever coated with phage
Author(s): Liling Fu; Suiqiong Li; Kewei Zhang; Z.-Y. Cheng; J.M. Barbaree
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Paper Abstract

Microcantilevers (MCs) as state-of-art sensor platforms have been widely investigated. We recently introduced a new type of MC, magnetostrictive microcantilever (MSMC), as high performance sensor platform. The MSMC is a remote/wireless sensor platform and exhibits a high quality merit factor in liquid. In this paper, a MSMC-based biosensor is developed for detecting B. anthracis spores in liquid, a potential biothreaten agent. The results demonstrated the advantages of MSMCs as a sensor platform. MSMCs with different sizes were fabricated and utilized in the experiments. The MSMCs were coated with the filamentous phage as a bio-recognition element to capture the B. anthracis spores. The phage-coated MSMCs as biosensors were exposed to cultures containing target spores with concentrations ranging from 5 * 104 spores/mL to 5 * 108 spores/mL. The resonance frequency of the MSMC sensors in cultures was monitored in a real-time manner. The results showed that for MSMCs of 2.8 mm * 1.0 mm * 35 &mgr;m and with 1.4 mm * 0.8 mm * 35 &mgr;m have a detection limit of 105 and 104 spores/mL, respectively.

Paper Details

Date Published: 10 May 2007
PDF: 9 pages
Proc. SPIE 6556, Micro (MEMS) and Nanotechnologies for Defense and Security, 655619 (10 May 2007); doi: 10.1117/12.721004
Show Author Affiliations
Liling Fu, Auburn Univ. (United States)
Suiqiong Li, Auburn Univ. (United States)
Kewei Zhang, Auburn Univ. (United States)
Z.-Y. Cheng, Auburn Univ. (United States)
J.M. Barbaree, Auburn Univ. (United States)

Published in SPIE Proceedings Vol. 6556:
Micro (MEMS) and Nanotechnologies for Defense and Security
Thomas George; Zhongyang Cheng, Editor(s)

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