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Proceedings Paper

Spectroscopic characterization of biological agents using FTIR, normal Raman and surface-enhanced Raman spectroscopies
Author(s): Tatiana Luna-Pineda; Kristina Soto-Feliciano; Edwin De La Cruz-Montoya; Leonardo C. Pacheco Londoño; Carlos Ríos-Velázquez; Samuel P. Hernández-Rivera
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Paper Abstract

FTIR, Raman spectroscopy and Surface Enhanced Raman Scattering (SERS) requires a minimum of sample allows fast identification of microorganisms. The use of this technique for characterizing the spectroscopic signatures of these agents and their stimulants has recently gained considerable attention due to the fact that these techniques can be easily adapted for standoff detection from considerable distances. The techniques also show high sensitivity and selectivity and offer near real time detection duty cycles. This research focuses in laying the grounds for the spectroscopic differentiation of Staphylococcus spp., Pseudomonas spp., Bacillus spp., Salmonella spp., Enterobacter aerogenes, Proteus mirabilis, Klebsiella pneumoniae, and E. coli, together with identification of their subspecies. In order to achieve the proponed objective, protocols to handle, cultivate and analyze the strains have been developed. Spectroscopic similarities and marked differences have been found for Spontaneous or Normal Raman spectra and for SERS using silver nanoparticles have been found. The use of principal component analysis (PCA), discriminate factor analysis (DFA) and a cluster analysis were used to evaluate the efficacy of identifying potential threat bacterial from their spectra collected on single bacteria. The DFA from the bacteria Raman spectra show a little discrimination between the diverse bacterial species however the results obtained from the SERS demonstrate to be high discrimination technique. The spectroscopic study will be extended to examine the spores produced by selected strains since these are more prone to be used as Biological Warfare Agents due to their increased mobility and possibility of airborne transport. Micro infrared spectroscopy as well as fiber coupled FTIR will also be used as possible sensors of target compounds.

Paper Details

Date Published: 27 April 2007
PDF: 11 pages
Proc. SPIE 6554, Chemical and Biological Sensing VIII, 65540K (27 April 2007); doi: 10.1117/12.720338
Show Author Affiliations
Tatiana Luna-Pineda, Univ. of Puerto Rico Mayagüez (United States)
Kristina Soto-Feliciano, Univ. of Puerto Rico Mayagüez (United States)
Edwin De La Cruz-Montoya, Univ. of Puerto Rico Mayagüez (United States)
Leonardo C. Pacheco Londoño, Univ. of Puerto Rico Mayagüez (United States)
Carlos Ríos-Velázquez, Univ. of Puerto Rico Mayagüez (United States)
Samuel P. Hernández-Rivera, Univ. of Puerto Rico Mayagüez (United States)

Published in SPIE Proceedings Vol. 6554:
Chemical and Biological Sensing VIII
Augustus W. Fountain, Editor(s)

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