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Proceedings Paper

High sensitivity detection of bacteria by surface plasmon resonance enhanced common path interferometry
Author(s): Charles Greef; Viatcheslav Petropavlovskikh; Oyvind Nilsen; Bilge Hacioglu; Boris Khattatov; John Hall
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Paper Abstract

Real time monitoring of biowarfare agents (BWA) for military and civilian protection remains a high priority for homeland security and battlefield readiness. Available devices have adequate sensitivity, but the detection modules have limited periods of deployment, require frequent maintenance, employ single-use disposable components, and have limited multiplexing capability. Surface Plasmon Resonance enhanced Common Path Interferometry (SPR-CPI) is a label-free, high sensitivity biomolecular interaction measurement technology that allows multiplexed real-time measurement of biowarfare agents, including small molecules, proteins, and microbes. The technology permits continuous operation in a field-deployable detection module of an integrated BWA monitoring system. SPR-CPI measures difference in phase shift of polarized light reflected from the transducer interface caused by changes in refractive index induced by biomolecular interactions. The measurement is performed on a discrete 2-dimensional area functionalized with biomolecule capture reagents in a microarray format, allowing simultaneous measurement of up to 100 separate analytes. Output consists of simultaneous voltage measurements proportional to the phase differences resulting from the refractive index changes and is automatically processed and displayed graphically or delivered to a decision making algorithm. This enables a fully automatic field-deployable detection system capable of integration into existing modular BWA detection systems. Proof-of-concept experiments on surrogate models of anticipated BWA threats have demonstrated utility. Efforts are in progress for full development and deployment of the device.

Paper Details

Date Published: 26 April 2007
PDF: 7 pages
Proc. SPIE 6554, Chemical and Biological Sensing VIII, 65540L (26 April 2007); doi: 10.1117/12.719409
Show Author Affiliations
Charles Greef, AlphaSniffer, LLC (United States)
Viatcheslav Petropavlovskikh, AlphaSniffer, LLC (United States)
Oyvind Nilsen, AlphaSniffer, LLC (United States)
Bilge Hacioglu, AlphaSniffer, LLC (United States)
Boris Khattatov, AlphaSniffer, LLC (United States)
John Hall, Hall Stable Lasers, LLC (United States)

Published in SPIE Proceedings Vol. 6554:
Chemical and Biological Sensing VIII
Augustus W. Fountain, Editor(s)

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