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Proceedings Paper

UV LED fiber optic detection system for DNA and protein
Author(s): Mathias Belz; Felix A. Klein; Heidi Habhegger
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Paper Abstract

Concentrations of DNA and proteins are traditionally detected at 260/280nm using laboratory spectrophotometers. Recently, AlGaN/GaN ultraviolet Light Emitting Diodes (LED) became available in the 250 nm to 350 nm wavelength region. An inexpensive fiber optic detection system based on these UV LEDs and photodiodes has been developed. It allows concentration measurements of such popular biochemistry samples. Measurement stability and noise will be discussed. The performance of the system in comparison to a standard spectrophotometer will be evaluated. In particular, the effect of decreasing the spectral resolution from usually used 2-3 nm to 10-20 nm Full Width Half Maximum (FWHM) is simulated and experimentally confirmed.

Paper Details

Date Published: 15 February 2007
PDF: 8 pages
Proc. SPIE 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII, 64330H (15 February 2007); doi: 10.1117/12.717552
Show Author Affiliations
Mathias Belz, World Precision Instruments, Inc. (United States)
Felix A. Klein, Fachhochschule Giessen-Friedberg (Germany)
Heidi Habhegger, World Precision Instruments, Inc. (United States)


Published in SPIE Proceedings Vol. 6433:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII
Israel Gannot, Editor(s)

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