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Proceedings Paper

Role of pH in the appearance of the fluorescent state of chromo protein asCP595 and its mutant KFP
Author(s): Alexander L. Rusanov; Nadya Zubova; Alexander P. Savitsky
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Paper Abstract

At the present time the phenomenon of "fluorescence kindling," which is typical of asCP595 protein and some of its mutants, is of great interest for the studies of intracellular traffic. The range of changing of intracellular pH may be rather wide, however, the effect of pH on the fluorescent state of this protein is poorly investigated. Our studies have revealed that the fluorescence intensity of asCP595 increases significantly in alkaline conditions. In addition, the observed change in the position of excitation, emission, and absorption maxima indicates the appearance of new spectral forms of the protein. These conformers are characteristics of new fluorescent states. The changes in the absorption spectrum are indicative of a new dark form of the protein at alkaline pH.

Paper Details

Date Published: 14 February 2007
PDF: 7 pages
Proc. SPIE 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV, 644917 (14 February 2007); doi: 10.1117/12.717411
Show Author Affiliations
Alexander L. Rusanov, M.V. Lomonosov Moscow State Univ. (Russia)
A.N. Bach Institute of Biochemistry (Russia)
Nadya Zubova, A.N. Bach Institute of Biochemistry (Russia)
Alexander P. Savitsky, M.V. Lomonosov Moscow State Univ. (Russia)
A.N. Bach Institute of Biochemistry (Russia)


Published in SPIE Proceedings Vol. 6449:
Genetically Engineered and Optical Probes for Biomedical Applications IV
Samuel Achilefu; Alexander Pavlovich Savitsky; Rebekka M. Wachter; Darryl J. Bornhop; Ramesh Raghavachari, Editor(s)

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