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Proceedings Paper

Advances in intravital microscopy for monitoring cell flow dynamics in vivo
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Paper Abstract

The use of small animals in intravital optical microscopy is a well-established experimental model to study blood microcirculation in vivo. Recent advances in cell biology and optical techniques (e.g., lasers, CCD cameras, software, etc.) provide the basis for significant improvements with in vivo imaging. This review summarizes the latest achievements in this specific area focusing on the development of modern optical and biological platforms. This includes in vivo real time monitoring of individual cells in the context of blood flow, super-sensitive fluorescence imaging, high-speed cell imaging and light scattering techniques. The capability of these platforms has been demonstrated in live animal models (e.g., mouse and rat ear, rat mesentery, and others) for real-time monitoring of individual blood cell properties (e.g., size and shape), cell trafficking, cell-cell interactions (e.g., aggregation in flow or adhesion to vessel walls), and blood flow viscosity. Future applications are discussed including in vivo early diagnosis of disease and monitoring cellular responses to environmental and therapeutic interventions.

Paper Details

Date Published: 7 February 2007
PDF: 15 pages
Proc. SPIE 6436, Complex Dynamics and Fluctuations in Biomedical Photonics IV, 64360D (7 February 2007); doi: 10.1117/12.716636
Show Author Affiliations
Vyacheslav Kalchenko, Weizmann Institute of Science (Israel)
Alon Harmelin, Weizmann Institute of Science (Israel)
Ilya Fine, Elfi Tech Ltd. (Israel)
Vladimir Zharov, Arkansas Cancer Research Ctr. ,Univ. of Arkansas for Medical Sciences (United States)
Ekaterina Galanzha, Arkansas Cancer Research Ctr. ,Univ. of Arkansas for Medical Sciences (United States)
Research-Educational Institute of Optics and Biophotonics, Saratov State Univ. (Russia)
Valery Tuchin, dResearch-Educational Institute of Optics and Biophotonics, Saratov State Univ. (Russia)


Published in SPIE Proceedings Vol. 6436:
Complex Dynamics and Fluctuations in Biomedical Photonics IV
Valery V. Tuchin, Editor(s)

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