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Proceedings Paper

A novel surface modification approach for protein and cell microarrays
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Paper Abstract

Tissue engineering and stem cell technologies have led to a rapidly increasing interest in the control of the behavior of mammalian cells growing on tissue culture substrates. Multifunctional polymer coatings can assist research in this area in many ways, for example, by providing low non-specific protein adsorption properties and reactive functional groups at the surface. The latter can be used for immobilization of specific biological factors that influence cell behavior. In this study, glass slides were coated with copolymers of glycidyl methacrylate (GMA) and poly(ethylene glycol) methacrylate (PEGMA). The coatings were prepared by three different methods based on dip and spin coating as well as polymer grafting procedures. Coatings were characterized by X-ray photoelectron spectroscopy, surface sensitive infrared spectroscopy, ellipsometry and contact angle measurements. A fluorescently labelled protein was deposited onto reactive coatings using a contact microarrayer. Printing of a model protein (fluorescein labeled bovine serum albumin) was performed at different protein concentrations, pH, temperature, humidity and using different micropins. The arraying of proteins was studied with a microarray scanner. Arrays printed at a protein concentration above 50 μg/mL prepared in pH 5 phosphate buffer at 10°C and 65% relative humidity gave the most favourable results in terms of the homogeneity of the printed spots and the fluorescence intensity.

Paper Details

Date Published: 9 January 2007
PDF: 12 pages
Proc. SPIE 6413, Smart Materials IV, 64130Z (9 January 2007); doi: 10.1117/12.715172
Show Author Affiliations
Mahaveer D Kurkuri, Flinders Univ. (Australia)
Chantelle Driever, CSIRO Molecular and Health Technologies (Australia)
Helmut W. Thissen, CSIRO Molecular and Health Technologies (Australia)
Nicholas H. Voelcker, Flinders Univ. (Australia)
CSIRO Molecular and Health Technologies (Australia)

Published in SPIE Proceedings Vol. 6413:
Smart Materials IV
Nicolas H. Voelcker, Editor(s)

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