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Proceedings Paper

Lab-on-a-chip platforms based on highly sensitive nanophotonic Si biosensors for single nucleotide DNA testing
Author(s): J. Sánchez del Río; L. G. Carrascosa; F. J. Blanco; M. Moreno; J. Berganzo; A. Calle; C. Domínguez; L. M. Lechuga
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Paper Abstract

In order to solve the drawbacks of sensitivity and portability in optical biosensors we have developed ultrasensitive and miniaturized photonic silicon sensors able to be integrated in a "lab-on-a-chip" microsystem platform. The sensors are integrated Mach-Zehnder interferometers based on TIR optical waveguides (Si/SiO2/Si3N4) of micro/nanodimensions. We have applied this biosensor for DNA testing and for detection of single nucleotide polymorphisms at BRCA-1 gene, involved in breast cancer development, without target labeling. The oligonucleotide probe is immobilized by covalent attachment to the sensor surface through silanization procedures. The hybridization was performed for different DNA target concentrations showing a lowest detection limit at 10 pM. Additionally, we have detected the hybridization of different concentrations of DNA target with two mismatching bases corresponding to a mutation of the BRCA-1 gene. Following the way of the lab-on-a-chip microsystem, integration with the microfluidics has been achieved by using a novel fabrication method of 3-D embedded microchannels using the polymer SU-8 as structural material. The optofluidic chip shows good performances for biosensing.

Paper Details

Date Published: 14 February 2007
PDF: 9 pages
Proc. SPIE 6477, Silicon Photonics II, 64771B (14 February 2007); doi: 10.1117/12.713977
Show Author Affiliations
J. Sánchez del Río, Microelectronics National Ctr. (Spain)
L. G. Carrascosa, Microelectronics National Ctr. (Spain)
F. J. Blanco, IKERLAN S.Coop. (Spain)
M. Moreno, Microelectronics National Ctr. (Spain)
J. Berganzo, IKERLAN S.Coop. (Spain)
A. Calle, Microelectronics National Ctr. (Spain)
C. Domínguez, Microelectronics National Ctr. (Spain)
L. M. Lechuga, Microelectronics National Ctr. (Spain)


Published in SPIE Proceedings Vol. 6477:
Silicon Photonics II
Joel A. Kubby; Graham T. Reed, Editor(s)

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