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Proceedings Paper

Fluorescence intermittency and spectral shifts of single bio-conjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy
Author(s): HyeongGon Kang; Mathew M. Maye; Dmytro Nykypanchuk; Matthew Clarke; Peter Yim; Jeffrey Krogmeier; Kimberly Briggman; Oleg Gang; Jeeseong Hwang
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Paper Abstract

We have fabricated a combined measurement system capable of confocal microscopy and fluorescence spectroscopy to simultaneously evaluate multiple optical characteristics of single fluorescent nanocrystals. The single particle detection sensitivity is demonstrated by simultaneously measuring the dynamic excitation-time-dependent fluorescence intermittency and the emission spectrum of single cadmium selenide/zinc sulfide (CdSe/ZnS) nanocrystals (quantum dots, QDs). Using this system, we are currently investigating the optical characteristics of single QDs, the surface of which are conjugated with different ligands, such as trioctylphosphine oxide (TOPO), mercaptoundecanoicacid (MDA), and amine modified DNA (AMDNA). In this paper, we present the progress of our measurements of the time-dependent optical characteristics (fluorescence intermittency, photostability, and spectral diffusion) of single MDA-QDs and AMDNA-MDA-QDs in air in an effort to understand the effects of surface-conjugated biomolecules on the optical characteristics at single QD sensitivities.

Paper Details

Date Published: 7 February 2007
PDF: 9 pages
Proc. SPIE 6430, Advanced Biomedical and Clinical Diagnostic Systems V, 643024 (7 February 2007); doi: 10.1117/12.713934
Show Author Affiliations
HyeongGon Kang, National Institute of Standards and Technology (United States)
Mathew M. Maye, Brookhaven National Lab. (United States)
Dmytro Nykypanchuk, Brookhaven National Lab. (United States)
Matthew Clarke, National Institute of Standards and Technology (United States)
Peter Yim, National Institute of Standards and Technology (United States)
Jeffrey Krogmeier, National Institute of Standards and Technology (United States)
Kimberly Briggman, National Institute of Standards and Technology (United States)
Oleg Gang, Brookhaven National Lab. (United States)
Jeeseong Hwang, National Institute of Standards and Technology (United States)


Published in SPIE Proceedings Vol. 6430:
Advanced Biomedical and Clinical Diagnostic Systems V
Ramesh Raghavachari; Tuan Vo-Dinh; Warren S. Grundfest; David A. Benaron; Gerald E. Cohn, Editor(s)

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