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Proceedings Paper

Methods and instruments for continuous-flow PCR on a chip
Author(s): Claudia Gärtner; Richard Klemm; Holger Becker
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Paper Abstract

PCR is the most commonly used method to identify DNA segments. Several methods have been used to miniaturize PCR and perform it in a microfluidic chip. A unique approach is the continuous-flow PCR, where the conventional thermocycling is replaced by pumping the sample through a channel which meanders over stationary temperature zones, allowing fast temperature changes in the sample due to the low thermal mass as well as a continuous production of PCR products. In this paper we present a system comprising a polymer microfluidic chip, a thermocycler unit and the protocols necessary to perform fast continuous-flow PCR including experimental results in comparison with a conventional PCR system.

Paper Details

Date Published: 22 January 2007
PDF: 8 pages
Proc. SPIE 6465, Microfluidics, BioMEMS, and Medical Microsystems V, 646502 (22 January 2007); doi: 10.1117/12.713797
Show Author Affiliations
Claudia Gärtner, microfluidic ChipShop GmbH (Germany)
Richard Klemm, Fraunhofer Institute for Applied Optics and Precision Mechanics (Germany)
Holger Becker, microfluidic ChipShop GmbH (Germany)

Published in SPIE Proceedings Vol. 6465:
Microfluidics, BioMEMS, and Medical Microsystems V
Ian Papautsky; Wanjun Wang, Editor(s)

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