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Proceedings Paper

Imaging ligand-gated ion channels with quantum dots
Author(s): I. D. Tomlinson; Rebecca L. Orndorff; Hélène Gussin; John N. Mason; Randy D. Blakely; David R. Pepperberg; Sandra J. Rosenthal
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Paper Abstract

In this paper we report two different methodologies for labeling ligand-gated receptors. The first of these builds upon our earlier work with serotonin conjugated quantum dots and our studies with pegilated quantum dots to reduce non specific binding. In this approach a pegilated derivative of muscimol was synthesized and attached via an amide linkage to quantum dots coated in an amphiphillic polymer derivative of poly acrylamide. These conjugates were used to image the GABAC receptor in oocytes. An alternative approach was used to image tissue sections to study nicotinic acetylcholine receptors in the neuro muscular junction with biotinylated Bungerotoxin and streptavidin coated quantum dots.

Paper Details

Date Published: 27 February 2007
PDF: 9 pages
Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 64480X (27 February 2007); doi: 10.1117/12.713696
Show Author Affiliations
I. D. Tomlinson, Vanderbilt Univ. (United States)
Rebecca L. Orndorff, Vanderbilt Univ. (United States)
Hélène Gussin, Univ. of Illinois at Chicago (United States)
John N. Mason, Vanderbilt Univ. (United States)
Randy D. Blakely, Vanderbilt Univ. (United States)
David R. Pepperberg, Univ. of Illinois at Chicago (United States)
Sandra J. Rosenthal, Vanderbilt Univ. (United States)

Published in SPIE Proceedings Vol. 6448:
Colloidal Quantum Dots for Biomedical Applications II
Marek Osinski; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

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