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Proceedings Paper

Methods for monitoring and imaging nanoparticles in cells
Author(s): Dmitri O. Lapotko; Ekaterina Y. Lukianova; Sergey A. Chizhik
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Paper Abstract

Successful targeting nanoparticles (NP) to specific cells requires reliable feedback about NP accumulation in cells. This task is a challenge for all optical methods due the size of NP and diffraction limit of optical devices. We modified several microscopy-based techniques for imaging and measuring NP in individual cells: photothermal, fluorescent, electron and atomic-force microscopies and flow cytometry. All those techniques were applied for quantitative analysis and imaging of interaction of gold NP (10 and 30 nm) with living tumor cells. Based on experimental results we performed comparison of all methods in terms of sensitivity, speed, sample requirements etc. We have found that standard microscopes may detect NP and their clusters in individual living cells through imaging NP-related thermal, fluorescent and other phenomena.

Paper Details

Date Published: 10 February 2007
PDF: 10 pages
Proc. SPIE 6447, Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV, 644703 (10 February 2007); doi: 10.1117/12.712855
Show Author Affiliations
Dmitri O. Lapotko, Lykov Heat and Mass Transfer Institute (Belarus)
Ekaterina Y. Lukianova, Lykov Heat and Mass Transfer Institute (Belarus)
Sergey A. Chizhik, Lykov Heat and Mass Transfer Institute (Belarus)


Published in SPIE Proceedings Vol. 6447:
Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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