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Proceedings Paper

Wide-field two-photon microscopy: features and advantages for biomedical applications
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Paper Abstract

We describe a simple fluorescence microscope based on wide-field two-photon excitation. While still taking advantage of some inherent properties of non-linear (two-photon) microscopy, such as increased penetration depth through tissue and reduced phototoxicity, this approach provides video frame rate imaging, can be easily coupled to fluorescence spectral and lifetime detection modules, and makes efficient use of the high average power currently available from ultrashort pulsed lasers. For a standard histopathology specimen, we were able to identify different structures based on spectral and fluorescence lifetime detection and analysis. We examined the use of 200fs and 2ps pulses from Spectra Physics MaiTai and Tsunami lasers, respectively, with average power ranging from 50mW to 500mW.

Paper Details

Date Published: 19 February 2007
PDF: 8 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64411B (19 February 2007); doi: 10.1117/12.711603
Show Author Affiliations
S. Wachsmann-Hogiu, Cedars-Sinai Medical Ctr. (United States)
J. Y. Hwang, Cedars-Sinai Medical Ctr. (United States)
Univ. of Southern California, Los Angeles (United States)
E. Lindsley, Cedars-Sinai Medical Ctr. (United States)
D. L. Farkas, Cedars-Sinai Medical Ctr. (United States)
Univ. of Southern California, Los Angeles (United States)


Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

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