Share Email Print
cover

Proceedings Paper

Multimode optical imaging of small animals: development and applications
Author(s): J. Y. Hwang; C. Moffatt-Blue; O. Equils; M. Fujita; J. Jeong; N. M. Khazenzon; E. Lindsley; J. Ljubimova; A. G. Nowatzyk; D. L. Farkas; S. Wachsmann-Hogiu
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We present an optical system for small animal imaging that can combine various in vivo imaging modalities, including fluorescence (intensity and lifetime), spectral, and trans-illumination imaging. This system consists of light-tight box with ultrafast pulsed or cw laser light excitation, motorized translational and rotational stages, a telecentric lens for detection, and a cooled CCD camera that can be coupled to an ultrafast time-gated intensifier. All components are modular, making possible laser excitation at various wavelengths and pulse lengths, and signal detection in a variety of ways (multimode). Results of drug nanoconjugate carrier delivery studies in mice are presented. Conventional and spectrally-resolved fluorescence images reveal details of in vivo drug nanoconjugate carrier accumulation within the tumor region and several organs in real time. By multi-spectral image analysis of ex vivo specimens from the same mice, we were able to evaluate the extent and topology of drug nanoconjugate carrier distribution into specific organs and the tumor itself.

Paper Details

Date Published: 19 February 2007
PDF: 10 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 644105 (19 February 2007); doi: 10.1117/12.711582
Show Author Affiliations
J. Y. Hwang, Univ. of Southern California, Los Angeles (United States)
Cedars-Sinai Medical Ctr. (United States)
C. Moffatt-Blue, Cedars-Sinai Medical Ctr. (United States)
O. Equils, Cedars-Sinai Medical Ctr. (United States)
M. Fujita, Cedars-Sinai Medical Ctr. (United States)
J. Jeong, Univ. of Southern California, Los Angeles (United States)
Cedars-Sinai Medical Ctr. (United States)
N. M. Khazenzon, Cedars-Sinai Medical Ctr. (United States)
E. Lindsley, Cedars-Sinai Medical Ctr. (United States)
J. Ljubimova, Cedars-Sinai Medical Ctr. (United States)
A. G. Nowatzyk, Univ. of Southern California, Los Angeles (United States)
Cedars-Sinai Medical Ctr. (United States)
D. L. Farkas, Univ. of Southern California, Los Angeles (United States)
Cedars-Sinai Medical Ctr. (United States)
S. Wachsmann-Hogiu, Cedars-Sinai Medical Ctr. (United States)


Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

© SPIE. Terms of Use
Back to Top