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Proceedings Paper

Micro-photonic cylindrical waveguide based protein biosensor
Author(s): Sudhaprasanna K. Padigi; Kofi Aasante; Ravi K. K. Reddy; Vijay S. R. Kovvuri; Andres LaRosa; Shalini Prasad
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Paper Abstract

We have experimentally demonstrated the fabrication and the functioning of a rapidly prototyped optical cylindrical microcavity waveguide based biosensor. The device works on the principle of determination of the change to the light intensity of the input coupled light to the waveguide due to the interaction and binding of proteins to the cylindrical waveguide structure. The variation to the coupled light intensity is dependant on the nature of the protein i.e. its surface charge and the density of the proteins. This technique has been used to identify a specific protein biomarker associated with the identification of vulnerable coronary plaque -Myeloperoxidase (MPO). Detection sensitivity in the order of pg/ml has been demonstrated. The detection speed is in the order of seconds from the time of injection of the protein onto the sensor surface. The optical signature that is obtained to identify a protein is entirely dependant on the nature of adsorption of the protein on to the cylindrical cavity surfaces. This technique is a demonstration of detection of nanoscale proteins using a label free optical biosensor technique with unprecedented sensitivity.

Paper Details

Date Published: 2 March 2007
PDF: 10 pages
Proc. SPIE 6475, Integrated Optics: Devices, Materials, and Technologies XI, 647503 (2 March 2007); doi: 10.1117/12.711207
Show Author Affiliations
Sudhaprasanna K. Padigi, Portland State Univ. (United States)
Kofi Aasante, Portland State Univ. (United States)
Ravi K. K. Reddy, Portland State Univ. (United States)
Vijay S. R. Kovvuri, Portland State Univ. (United States)
Andres LaRosa, Portland State Univ. (United States)
Shalini Prasad, Portland State Univ. (United States)

Published in SPIE Proceedings Vol. 6475:
Integrated Optics: Devices, Materials, and Technologies XI
Yakov Sidorin; Christoph A. Waechter, Editor(s)

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