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Proceedings Paper

Beyond monochromatic light: three-dimensional confocal laser scanning microscopy using a supercontinuum source
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Paper Abstract

Confocal laser scanning microscopy (CLSM) has rapidly become an essential tool in the life sciences laboratory, enabling high-resolution and minimally intrusive optical sectioning of fluorescent samples. Most commercially available CLSM systems employ a gas laser, e.g. a Kr/Ar laser, to provide the excitation radiation. However, such lasers have several shortcomings, including the maintenance requirements, short lifetimes and high noise levels. To overcome these limitations, a light source for CLSM that is based on supercontinuum generation in photonic crystal fiber has been developed. This source provides the necessary wavelength range required to excite the widest possible variety of fluorophores. A novel method of extracting the desired wavelengths from the supercontinuum source using a digital micro-mirror device (DMD) is also described.

Paper Details

Date Published: 27 October 2006
PDF: 5 pages
Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472Q (27 October 2006); doi: 10.1117/12.710991
Show Author Affiliations
G. McConnell, Ctr. for Biophotonics, Univ. of Strathclyde (United Kingdom)
J. M. Girkin, Univ. of Strathclyde (United Kingdom)
S. Poland, Univ. of Strathclyde (United Kingdom)

Published in SPIE Proceedings Vol. 6047:
Fourth International Conference on Photonics and Imaging in Biology and Medicine
Kexin Xu; Qingming Luo; Da Xing; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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