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Proceedings Paper

Two-photon fluorescence anisotropy imaging
Author(s): Wei Li; Yi Wang; Hanrong Shao; Yonghong He; Hui Ma
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Paper Abstract

We have developed a novel method for imaging the fluorescence intensity and anisotropy by two-photon fluorescence microscopy and tested its capability in biological application. This method is applied to model sample including FITC and FITC-CD44 antibody solution and also FITC-CD44 stained cells. The fluorescence anisotropy (FA) of FITC-CD44ab solution is higher than the FITC solution with the same concentration. The fluorescence in cell sample has even higher FA than in solution because the rotation diffusion is restrained in membrane. The method is employed to study the effect of berberine a kind of Chinese medicine, on tumor metastasis. The results indicated that tumor cell membrane fluidity is decreasing with increasing the concentration of berberine in culture medium.

Paper Details

Date Published: 27 October 2006
PDF: 5 pages
Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472R (27 October 2006); doi: 10.1117/12.710987
Show Author Affiliations
Wei Li, Tsinghua Univ. (China)
Yi Wang, Tsinghua Univ. (China)
Hanrong Shao, Tsinghua Univ. (China)
Yonghong He, Tsinghua Univ. (China)
Hui Ma, Tsinghua Univ. (China)

Published in SPIE Proceedings Vol. 6047:
Fourth International Conference on Photonics and Imaging in Biology and Medicine
Kexin Xu; Qingming Luo; Da Xing; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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