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Proceedings Paper

Determining biomolecular structures by time-resolved fluorescence
Author(s): Olaf J. Rolinski; Aaron Hernandez-Santana; Duncan Graham
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Paper Abstract

We demonstrate a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.

Paper Details

Date Published: 27 October 2006
PDF: 6 pages
Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472N (27 October 2006); doi: 10.1117/12.710984
Show Author Affiliations
Olaf J. Rolinski, Univ. of Strathclyde (United Kingdom)
Aaron Hernandez-Santana, Univ. of Strathclyde (United Kingdom)
Duncan Graham, Univ. of Strathclyde (United Kingdom)


Published in SPIE Proceedings Vol. 6047:
Fourth International Conference on Photonics and Imaging in Biology and Medicine
Kexin Xu; Qingming Luo; Da Xing; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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