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Proceedings Paper

Monitoring apoptosis of TK-GFP-expressing ACC-M cells induced by ACV using FRET technique
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Paper Abstract

Apoptosis is an evolutionary conserved cellular process that plays an important role during development, but it is also involved in tissue homeostasis and in many diseases. To study the characteristics of suicide gene system of the herpes simplex virus thymidine kinase (HSV-tk) gene in tumor cells and explore the apoptosis phenomena in this system and its effect on the human adenoid cystic carcinoma line ACC-M cell, we detected apoptosis of CD3- (ECFP-CRS-DsRed) and TK-GFP-expressing ACC-M (ACC-M-TK-GFP-CD3) cells induced by acyclovir (ACV) using fluorescence resonance energy transfer (FRET) technique. CD3 is a FRET-based indicator for activity of caspase-3, which is composed of an enhanced cyan fluorescent protein, a caspase-3 sensitive linker, and a red fluorescent protein from Discosoma with efficient maturation property. FRET from ECFP to DsRed could be detected in normal ACC-M-TK-GFP-CD3 cells, and the FRET efficient was remarkably decreased and then disappeared during the cells apoptosis induced by ACV. It was due to the activated caspase-3 cleaved the CD3 fusion protein. In this study, the results suggested that the AVC-induced apoptosis of ACC-M-TK-GFP-CD3 cells was through caspase-3 pathway.

Paper Details

Date Published: 27 October 2006
PDF: 5 pages
Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472J (27 October 2006); doi: 10.1117/12.710962
Show Author Affiliations
Tao Xiong, Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
China Univ. of Geosciences (China)
Zhihong Zhang, Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Hubei Bioinformatics and Molecular Imaging Key Lab., Huazhong Univ. of Science and Technology (China)
Juqiang Lin, Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Jie Yang, Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Hubei Bioinformatics and Molecular Imaging Key Lab., Huazhong Univ. of Science and Technology (China)
Qingming Luo, Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Hubei Bioinformatics and Molecular Imaging Key Lab., Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 6047:
Fourth International Conference on Photonics and Imaging in Biology and Medicine
Kexin Xu; Qingming Luo; Da Xing; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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