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Proceedings Paper

An electrochemiluminescence non-PCR method for the detection of genetically modified organisms
Author(s): Jinfeng Liu; Da Xing; Debin Zhu
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Paper Abstract

An electrochemiluminescence non-PCR method has been developed for the detection of genetically modified organisms (GMOs) in crops. Genomic DNA of GMOs was digested with two restriction endonucleases (FOK I and BsrD I), and hybridized with three Ru(bpy)32+ (TBR)-labeled and one biotinylated probes. The hybridization products were captured onto streptavidin-coated paramagnetic beads, and detected by measuring the electrochemiluminescence (ECL) signal of the TBR label. Whether the tobaccos contain GM components was discriminated by detecting the ECL signal of CaMV35S promoter. The experiment results show that the detection limit for CaMV35S promoter is 100 fmol, and the GM components can be clearly identified in GM tobaccos. The ECL non-PCR method will provide a new means in GMOs detection due to its safety, simplicity and high efficiency.

Paper Details

Date Published: 27 October 2006
PDF: 7 pages
Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60473D (27 October 2006); doi: 10.1117/12.710937
Show Author Affiliations
Jinfeng Liu, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Debin Zhu, South China Normal Univ. (China)


Published in SPIE Proceedings Vol. 6047:
Fourth International Conference on Photonics and Imaging in Biology and Medicine
Kexin Xu; Qingming Luo; Da Xing; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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