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Proceedings Paper

The collagen structure of equine articular cartilage characterized using polarization-sensitive optical coherence tomography and non-linear microscopy
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Paper Abstract

Equine articular cartilage has been imaged using both polarization-sensitive optical coherence tomography (PS-OCT) and non-linear microscopy. PS-OCT has been used to spatially map the birefringence in the cartilage and we have found that in the vicinity of the lesion the images display a characteristic disruption in the regular birefringence bands shown by normal cartilage. We also note that significant (e.g. x2) variations in the apparent birefringence of samples taken from young (18 month) animals that otherwise appear visually homogeneous are found over spatial scales of a few millimeters. We have also imaged the cartilage using non-linear microscopy and compare the scans taken with second harmonic generation (SHG) light and the two photon fluorescence (TPF) light. SHG images collected using 800 nm excitation reveals the spatial distribution of collagen fibers, whilst TPF images clearly shows the distribution of intracellular and pericellular fluorophores.

Paper Details

Date Published: 27 October 2006
PDF: 8 pages
Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 604715 (27 October 2006); doi: 10.1117/12.710873
Show Author Affiliations
Jessica C. Mansfield, Univ. of Exeter (United Kingdom)
Nadya Ugryumova, Univ. of Exeter (United Kingdom)
Karen M. Knapp, Univ. of Exeter (United Kingdom)
Stephen J. Matcher, Univ. of Exeter (United Kingdom)


Published in SPIE Proceedings Vol. 6047:
Fourth International Conference on Photonics and Imaging in Biology and Medicine

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