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Proceedings Paper

Feasibility of chemiluminescence as photodynamic therapy dosimetor
Author(s): Yanfang Qin; Da Xing; Xueyun Zhong; Jin Zhou; Shiming Luo; Qun Chen
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Paper Abstract

Photodynamic therapy (PDT) utilizes light energy of a proper wavelength to activate a pre-administered photosensitizer in a target tissue to achieve a localized treatment effect. Current treatment protocol of photodynamic therapy (PDT) is defined by empirical values such as irradiation light fluence, fluence rate and the amount of administered photosensitizer. It is well known that Singlet oxygen is the most important cytotoxic agent responsible for PDT biological effects. An in situ monitoring of singlet oxygen production during PDT would provide a more accurate dosimeter for PDT. The presented study has investigated the feasibility of using Fhioresceinyl Cypridina Luciferin Analog (FCLA), a singlet oxygen specific chemiluminescence (CL) probe, as a dosimetric tool for PDT. Raji lymphoma cell suspensions were sensitized with Photofrin(R) of various concentrations and irradiated with 635 nm laser light at different fluence rates. FCLA-CL from singlet oxygen produced by the treatment was measured, in real time, with a photon multiplier tube (PMT) system, and linked to the cytotoxicity resulting from the treatment. We have observed that the CL intensity of FCLA is dependent on the PDT treatment parameters. After each PDT treatment and CL measurement, the irradiated cells were evaluated by MIT assay for their Viability. The results show that the cell viability is highly related to the accumulated CL. With 102 quencher, we confirmed that the CL was mainly related to PDT produced 102 The results suggest that the FCLA-CL system can be an effective means in measuring PDT 1O2 production and may provide an alternative dosimetry technique for PDT.

Paper Details

Date Published: 27 October 2006
PDF: 7 pages
Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60473B (27 October 2006); doi: 10.1117/12.710705
Show Author Affiliations
Yanfang Qin, Ji Nan Univ. (China)
South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Xueyun Zhong, Ji Nan Univ. (China)
Jin Zhou, Ji Nan Univ. (China)
Shiming Luo, Ji Nan Univ. (China)
Qun Chen, South China Normal Univ. (China)
HealthONE (United States)


Published in SPIE Proceedings Vol. 6047:
Fourth International Conference on Photonics and Imaging in Biology and Medicine
Kexin Xu; Qingming Luo; Da Xing; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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