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Proceedings Paper

A digital rat atlas of sectional anatomy
Author(s): Li Yu; Qian Liu; Xueling Bai; Yinping Liao; Qingming Luo; Hui Gong
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Paper Abstract

This paper describes a digital rat alias of sectional anatomy made by milling. Two healthy Sprague-Dawley (SD) rat weighing 160-180 g were used for the generation of this atlas. The rats were depilated completely, then euthanized by Co2. One was via vascular perfusion, the other was directly frozen at -85 °C over 24 hour. After that, the frozen specimens were transferred into iron molds for embedding. A 3% gelatin solution colored blue was used to fill the molds and then frozen at -85 °C for one or two days. The frozen specimen-blocks were subsequently sectioned on the cryosection-milling machine in a plane oriented approximately transverse to the long axis of the body. The surface of specimen-blocks were imaged by a scanner and digitalized into 4,600 x2,580 x 24 bit array through a computer. Finally 9,475 sectional images (arterial vessel were not perfused) and 1,646 sectional images (arterial vessel were perfused) were captured, which made the volume of the digital atlas up to 369.35 Gbyte. This digital rat atlas is aimed at the whole rat and the rat arterial vessels are also presented. We have reconstructed this atlas. The information from the two-dimensional (2-D) images of serial sections and three-dimensional (3-D) surface model all shows that the digital rat atlas we constructed is high quality. This work lays the foundation for a deeper study of digital rat.

Paper Details

Date Published: 27 October 2006
PDF: 7 pages
Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60470Y (27 October 2006); doi: 10.1117/12.710690
Show Author Affiliations
Li Yu, Hubei Bioinformatics and Molecular Imaging Key Lab., Huazhong Univ. of Science and Technology (China)
Qian Liu, Hubei Bioinformatics and Molecular Imaging Key Lab., Huazhong Univ. of Science and Technology (China)
Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Xueling Bai, Hubei Bioinformatics and Molecular Imaging Key Lab., Huazhong Univ. of Science and Technology (China)
Yinping Liao, Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Hubei Bioinformatics and Molecular Imaging Key Lab., Huazhong Univ. of Science and Technology (China)
Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Hui Gong, Hubei Bioinformatics and Molecular Imaging Key Lab., Huazhong Univ. of Science and Technology (China)
Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 6047:
Fourth International Conference on Photonics and Imaging in Biology and Medicine
Kexin Xu; Qingming Luo; Da Xing; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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