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Proceedings Paper

True-color nanosecond time-gated fluorescence imaging using a CCD detector
Author(s): A. C. Mitchell; E. J. Wall; C. G. Morgan
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Paper Abstract

We have developed a solid-state true-color charge-coupled (CCD) imager for nanosecond time-gated fluorescence imaging. Unlike intensifier-based imagers, the Peltier-cooled CCD imager is directly gated and hence has very low noise. The excitation source used with the imager is a compact diode-pumped nanosecond pulsed laser giving outputs at 532nm and 355nm with a variable repetition rate up to more than 10kHz. The operation of the new imager has been demonstrated using fluorescent samples that differ both in emission spectrum and in fluorescence decay time. Potential applications of the new technology are discussed including multiplexed detection of labels based on the combination of emission spectrum and fluorescence lifetime.

Paper Details

Date Published: 14 February 2007
PDF: 8 pages
Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430B (14 February 2007); doi: 10.1117/12.710566
Show Author Affiliations
A. C. Mitchell, Univ. of Salford (United Kingdom)
E. J. Wall, Univ. of Salford (United Kingdom)
C. G. Morgan, Univ. of Salford (United Kingdom)

Published in SPIE Proceedings Vol. 6443:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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