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Proceedings Paper

Retinal oxygen saturation evaluation by multi-spectral fundus imaging
Author(s): Bahram Khoobehi; Jinfeng Ning; Elise Puissegur; Kimberly Bordeaux; Madhusudhanan Balasubramanian; James Beach
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Paper Abstract

Purpose: To develop a multi-spectral method to measure oxygen saturation of the retina in the human eye. Methods: Five Cynomolgus monkeys with normal eyes were anesthetized with intramuscular ketamine/xylazine and intravenous pentobarbital. Multi-spectral fundus imaging was performed in five monkeys with a commercial fundus camera equipped with a liquid crystal tuned filter in the illumination light path and a 16-bit digital camera. Recording parameters were controlled with software written specifically for the application. Seven images at successively longer oxygen-sensing wavelengths were recorded within 4 seconds. Individual images for each wavelength were captured in less than 100 msec of flash illumination. Slightly misaligned images of separate wavelengths due to slight eye motion were registered and corrected by translational and rotational image registration prior to analysis. Numerical values of relative oxygen saturation of retinal arteries and veins and the underlying tissue in between the artery/vein pairs were evaluated by an algorithm previously described, but which is now corrected for blood volume from averaged pixels (n > 1000). Color saturation maps were constructed by applying the algorithm at each image pixel using a Matlab script. Results: Both the numerical values of relative oxygen saturation and the saturation maps correspond to the physiological condition, that is, in a normal retina, the artery is more saturated than the tissue and the tissue is more saturated than the vein. With the multi-spectral fundus camera and proper registration of the multi-wavelength images, we were able to determine oxygen saturation in the primate retinal structures on a tolerable time scale which is applicable to human subjects. Conclusions: Seven wavelength multi-spectral imagery can be used to measure oxygen saturation in retinal artery, vein, and tissue (microcirculation). This technique is safe and can be used to monitor oxygen uptake in humans. This work is original and is not under consideration for publication elsewhere.

Paper Details

Date Published: 29 March 2007
PDF: 6 pages
Proc. SPIE 6511, Medical Imaging 2007: Physiology, Function, and Structure from Medical Images, 65110B (29 March 2007); doi: 10.1117/12.710030
Show Author Affiliations
Bahram Khoobehi, Louisiana State Univ. Health Sciences Ctr. (United States)
Tulane Univ. (United States)
Jinfeng Ning, Tulane Univ. (United States)
Elise Puissegur, Tulane Univ. (United States)
Kimberly Bordeaux, Tulane Univ. (United States)
Madhusudhanan Balasubramanian, Louisiana State Univ. (United States)
James Beach, Univ. of Iceland (Iceland)


Published in SPIE Proceedings Vol. 6511:
Medical Imaging 2007: Physiology, Function, and Structure from Medical Images
Armando Manduca; Xiaoping P. Hu, Editor(s)

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