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Proceedings Paper

Attenuation of molecular function by multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALI) using green fluorescent protein
Author(s): Tetsuro Takamatsu
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Paper Abstract

Noninvasive and straightforward methods to inactivate selected proteins in the living cell with high spatiotemporal resolution are eagerly sought for elucidation of protein function in the post-genome-mapping era. Chromophore-assisted laser inactivation (CALI) facilitates inactivation of proteins by photochemically generated reactive oxygen species (ROS), but CALI using single-photon excitation thus far has presented several drawbacks, including its complex procedure, low efficiencies of inactivation with a certain chromophore, and photodamage effects. We here show that by application of multiphoton excitation to CALI using near-infrared femtosecond laser, enhanced green fluorescent protein (EGFP) can work as an effective chromophore for inactivation of a protein's function without nonspecific photodamage in the living cell.

Paper Details

Date Published: 13 March 2007
PDF: 8 pages
Proc. SPIE 6460, Commercial and Biomedical Applications of Ultrafast Lasers VII, 646004 (13 March 2007); doi: 10.1117/12.709235
Show Author Affiliations
Tetsuro Takamatsu, Kyoto Prefectural Univ. of Medicine (Japan)

Published in SPIE Proceedings Vol. 6460:
Commercial and Biomedical Applications of Ultrafast Lasers VII
Joseph Neev; Stefan Nolte; Alexander Heisterkamp; Christopher B. Schaffer, Editor(s)

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