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Proceedings Paper

Encapsulation of cell into monodispersed hydrogels on microfluidic device
Author(s): Chang-Hyoung Choi; Ji-Hye Lee; Hyun-Woo Shim; Nae-Rym Lee; Jae-Hoon Jung; Tae-Ho Yoon; Dong-Pyo Kim; Chang-Soo Lee
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Paper Abstract

In here, we present the microfluidic approach to produce monodispersed microbeads that will contain viable cells. The utilization of microfludics is helpful to synthesize monodispersed alginate hydrogels and in situ encapsulate cell into the generating hydrogels in microfludic device. First, the condition of formation of hydrogels in multiphase flows including oil, CaCl2, and alginate was optimized. Based on the preliminary survey, microfludic device could easily manipulate the size of alginate beads having narrow size distribution. The microfluidic method manipulates the size of hydrogel microbeads from 30 to 200um with a variation less than 2%. For the proof of concept of cell entrapment, the live yeast expressing green fluorescence protein is successfully encapsulated in microfluidic device.

Paper Details

Date Published: 14 December 2006
PDF: 4 pages
Proc. SPIE 6416, Biomedical Applications of Micro- and Nanoengineering III, 641613 (14 December 2006); doi: 10.1117/12.708548
Show Author Affiliations
Chang-Hyoung Choi, Chungnam National Univ. (South Korea)
Ji-Hye Lee, Chungnam National Univ. (South Korea)
Hyun-Woo Shim, Chungnam National Univ. (South Korea)
Nae-Rym Lee, Chungnam National Univ. (South Korea)
Jae-Hoon Jung, Chungnam National Univ. (South Korea)
Tae-Ho Yoon, Chungnam National Univ. (South Korea)
Dong-Pyo Kim, Chungnam National Univ. (South Korea)
Chang-Soo Lee, Chungnam National Univ. (South Korea)

Published in SPIE Proceedings Vol. 6416:
Biomedical Applications of Micro- and Nanoengineering III
Dan V. Nicolau, Editor(s)

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