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Proceedings Paper

Detection and identification of a water mixture of E. coli cells and B. subtilis spores with Raman chemical imaging microscopy
Author(s): Ashish Tripathi; Rabih E. Jabbour; Patrick J. Treado; Jason H. Neiss; Matthew P. Nelson; Janet L. Jensen; A. Peter Snyder
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Paper Abstract

Raman spectroscopy is being evaluated as a candidate technology for waterborne pathogen detection and the fidelity of the Raman spectra of microorganisms with respect to their differentiation at the single cell level are investigated. Individual entities are investigated in the microscope field of view (FOV) by Raman chemical imaging microscopy (RCIM). The size of a substance was not found to cause spectral confusion when collating individual entities in the FOV by multivariate principal components (PCA) and RCIM methods. Polystyrene (PS) beads in 1-3 micron sizes were collectively grouped together by PCA. Distilled and recipe tap water matrices produced the proper identification of the PS beads throughout the FOV, and all PS beads in a FOV were grouped together by PCA. A mixture of Gram-positive Bacillus atrophaeus spores and Gram-negative E. coli cells were differentiated and distinguished by RCIM.

Paper Details

Date Published: 26 April 2007
PDF: 9 pages
Proc. SPIE 6554, Chemical and Biological Sensing VIII, 65540J (26 April 2007); doi: 10.1117/12.707788
Show Author Affiliations
Ashish Tripathi, Science Applications International Corp. (United States)
Rabih E. Jabbour, Science Applications International Corp. (United States)
Patrick J. Treado, ChemImage Corp. (United States)
Jason H. Neiss, ChemImage Corp. (United States)
Matthew P. Nelson, ChemImage Corp. (United States)
Janet L. Jensen, Edgewood Chemical Biological Ctr. (United States)
A. Peter Snyder, Edgewood Chemical Biological Ctr. (United States)


Published in SPIE Proceedings Vol. 6554:
Chemical and Biological Sensing VIII
Augustus W. Fountain, Editor(s)

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