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Proceedings Paper

Photonic force microscopy of local tension at cell surface focal adhesions
Author(s): François Bordeleau; Judicaël Bessard; Normand Marceau; Yunlong Sheng
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Paper Abstract

The ability of cells to sustain mechanical stress is essential. It is however not very well understood how tension is expressed from the inside of the cell to the exterior. Here we show that these forces can be measured by photonic force microscopy (PFM), which is able to apply a force to cells and to probe their response to the physical stress. Our setup consists of an inverted microscope coupled with an optical trap from a 5W ND:YVO4 fiber laser. Forces are applied to the cell by the trap through a 5μm polystyrene bead coated with fibronectin, pre-incubated with cells to allow bead attachment and creation of focal adhesions. The reaction of the cell is monitored by sensing the position of the bead relative to the trap center by a quadrant photodiode, when the bead is in an equilibrium state between the photonics forces and the membrane elasticity and cell stiffness. The detection system is calibrated using a piezo nano-positioner and thermal noise analysis. We observed increased deformation of H4 cells treated with cytocholasin D, which disrupts the actin microfilaments. This observation is correlated to an overall decrease in the force by the photonics force microscope. Our results suggest that cell stiffness can be assessed by the PFM, which allows quantification of a tension within cells with sufficient precision.

Paper Details

Date Published: 8 September 2006
PDF: 10 pages
Proc. SPIE 6343, Photonics North 2006, 63430A (8 September 2006); doi: 10.1117/12.706573
Show Author Affiliations
François Bordeleau, Univ. Laval (Canada)
Judicaël Bessard, Univ. Laval (Canada)
Normand Marceau, Ctr. de recherche de l'Hôtel-Dieu de Québec (Canada)
Yunlong Sheng, Univ. Laval (Canada)

Published in SPIE Proceedings Vol. 6343:
Photonics North 2006
Pierre Mathieu, Editor(s)

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