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Proceedings Paper

Biological crystal alignment using image processing
Author(s): Kazimierz J. Gofron; Krzysztof Lazarski; Michael Molitsky; Andrzej Joachimiak
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Paper Abstract

Crystal location and alignment to the x-ray beam is an enabling technology necessary for automation of the macromolecular crystallography at synchrotron beamlines. In a process of crystal structure determination, a small size x-ray synchrotron beam with FWHM as small as 70 μm (bending magnet beamlines) and 20 μm (undulator beamlines) is focused at or downstream of the crystal sample. Protein crystals used in structure determination become smaller and approach 50 μm or less, and need to be precisely placed in the focused x-ray beam. At the Structural Biology Center the crystals are mounted on a goniostat, allowing precise crystal xyz positioning and rotations. One low and two high magnification cameras integrated into synchrotron beamline permit imaging of the crystal mounted on a goniostat. The crystals are held near liquid nitrogen temperatures using cryostream to control secondary radiation damage. Image processing techniques are used for automatic and precise placing of protein crystals in synchrotron beam. Here we are discussing automatic crystal centering process considered for Structure Biology Center utilizing several image processing techniques.

Paper Details

Date Published: 30 August 2006
PDF: 10 pages
Proc. SPIE 6312, Applications of Digital Image Processing XXIX, 63121W (30 August 2006); doi: 10.1117/12.706125
Show Author Affiliations
Kazimierz J. Gofron, Argonne National Lab. (United States)
Krzysztof Lazarski, Argonne National Lab. (United States)
Michael Molitsky, Argonne National Lab. (United States)
Andrzej Joachimiak, Argonne National Lab. (United States)

Published in SPIE Proceedings Vol. 6312:
Applications of Digital Image Processing XXIX
Andrew G. Tescher, Editor(s)

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