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Proceedings Paper

Integrated biophotonic hybridization sensor based on chitosan-mediated assembly
Author(s): Vlad Badilita; Michael Powers; Stephan Koev; Hyunmin Yi; Gregory Payne; Reza Ghodssi
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Paper Abstract

We present the use of the polysaccharide chitosan for immobilizing biomolecules on microfabricated device surfaces. The main advantages of chitosan are its abundance of primary amine groups and its ability to be electrodeposited. Biomolecules are easily attached to chitosan's amines by standard glutaraldehyde chemistry. The electrodeposition of chitosan allows accurate spatial and temporal control of biomolecule placement. We have used this biofunctionalization approach to develop a biophotonic hybridization sensor. Here we present for the first time probe DNA functionalization of the chitosan interface and hybridization detection using fluorescently labeled target DNA and integrated optical waveguides.

Paper Details

Date Published: 12 February 2007
PDF: 5 pages
Proc. SPIE 6464, MEMS/MOEMS Components and Their Applications IV, 646404 (12 February 2007); doi: 10.1117/12.705612
Show Author Affiliations
Vlad Badilita, Univ. of Maryland, College Park (United States)
Michael Powers, Univ. of Maryland, College Park (United States)
Stephan Koev, Univ. of Maryland, College Park (United States)
Hyunmin Yi, Univ. of Maryland, College Park (United States)
Tufts Univ. (United States)
Gregory Payne, Univ. of Maryland, College Park (United States)
Reza Ghodssi, Univ. of Maryland, College Park (United States)


Published in SPIE Proceedings Vol. 6464:
MEMS/MOEMS Components and Their Applications IV
Srinivas A. Tadigadapa; Reza Ghodssi; Albert K. Henning, Editor(s)

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