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Proceedings Paper

The influence of NIR femtosecond laser radiation on the viability of 3D stem cell clusters and tumor spheroids
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Paper Abstract

Adult human and rat pancreas stem cells as well as tumor spheroids were irradiated with femtosecond laser pulses in the near infrared (NIR) spectral range at high transient GW/cm2 and TW/cm2 intensities. The cellular response to the laser exposure was probed by the detection of modifications of NAD(P)H autofluorescence, the formation of reactive oxygen species (ROS) and DNA strand breaks (TUNEL-assay), and viability (live/dead test). The results confirm that long-term scanning of stem cells can be performed at appropriate laser exposure parameters without a measurable impact on the cellular metabolism and vitality. In addition, it was proven that a targeted inactivation of a particular single stem cells or a single tumour cell inside a 3D cell cluster using single point illumination at TW/cm2 laser intensities can be performed without affecting neighbouring cells. Therefore multiphoton microscopes can be considered as biosafe tools for long-term analysis of stem cells as well as highly precise optical knocking out of single cells within cell clusters and tissues.

Paper Details

Date Published: 10 February 2007
PDF: 5 pages
Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421Z (10 February 2007); doi: 10.1117/12.702586
Show Author Affiliations
Aisada Uchugonova, Fraunhofer Institute of Biomedical Technology (Germany)
Iris Riemann, Fraunhofer Institute of Biomedical Technology (Germany)
Frank Stracke, Fraunhofer Institute of Biomedical Technology (Germany)
Erwin Gorjup, Fraunhofer Institute of Biomedical Technology (Germany)
Ronan LeHarzic, JenLab GmbH (Germany)
Karsten König, Fraunhofer Institute of Biomedical Technology (Germany)


Published in SPIE Proceedings Vol. 6442:
Multiphoton Microscopy in the Biomedical Sciences VII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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