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Proceedings Paper

Applications of rigid and flexible GRIN-endoscopes
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Paper Abstract

Multiphoton autofluorescence imaging became an important technique for minimal invasive examination of cells in biological tissue. Rigid and flexible endoscopes based on gradient index lenses (GRIN-lenses) extend this minimalinvasive technique to deep lying cell layers, inner body and specimens, difficult to access. In the rigid endoscope, a GRIN-lens overcomes the limited depth range, given by the working distance of the microscope objective. The focus of the conventional laser scanning tomography is reproduced tens of millimeters in the specimen under study by the GRIN-lens (diameter 1.8 and 3 &mgr;m). We will present images of fluorescent beads, proteins cells and skin tissue, as well as first in vivo measurements on human skin. The autofluorescence signal stems from the endogenous fluorophore elastin and SHG from collagen. The flexible endoscope dispenses completely the need of a microscope next to the specimen of interest. The excitation laser pulses is delivered via a well-characterized photonic crystal fiber and subsequently focused by a newly designed GRIN-lens system. The fluorescence, also transferred by a fiber is detected by a PMT detector. We will show the excellent imaging qualities of a newly developed GRIN-lens system with high-resolution images of proteins, cells and plant tissue and give an out-look on multiphoton endoscopy.

Paper Details

Date Published: 15 February 2007
PDF: 7 pages
Proc. SPIE 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII, 64330N (15 February 2007); doi: 10.1117/12.702413
Show Author Affiliations
Selma Schenkl, Saarland Univ. (Germany)
Fraunhofer Institute of Biomedical Technology (Germany)
Alexander Ehlers, Saarland Univ. (Germany)
Fraunhofer Institute of Biomedical Technology (Germany)
Iris Riemann, Fraunhofer Institute of Biomedical Technology (Germany)
Bernhard Messerschmidt, GrinTech GmbH (Germany)
Rainer Bückle, Jenlab GmbH (Germany)
Karsten König, Saarland Univ. (Germany)
Fraunhofer Institute of Biomedical Technology (Germany)


Published in SPIE Proceedings Vol. 6433:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII
Israel Gannot, Editor(s)

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