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Proceedings Paper

Optical nano-injection into cells and 3D stem cell clusters via a NIR femtosecond laser
Author(s): Iris Riemann; Frank Stracke; Aisada Uchugonova; Sven Martin; Rainer Bückle; Karsten König
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Paper Abstract

Multiphoton optoporation of vital cells was performed using a femtosecond pulsed laser in the near infrared (NIR). Exogenous materials such as macromolecules and exogenes were transported into the targets via laser assisted transient opening of the cell membrane. This method is also appropriate for nanoprocessing and optoporation inside 3D stem cell structures without photodestructive collateral effects which was confirmed with TUNEL-assay (DNA strand breaks) and tests for reactive oxygen species (ROS).

Paper Details

Date Published: 10 February 2007
PDF: 6 pages
Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421F (10 February 2007); doi: 10.1117/12.702399
Show Author Affiliations
Iris Riemann, Fraunhofer Institute of Biomedical Technology (Germany)
Frank Stracke, Fraunhofer Institute of Biomedical Technology (Germany)
Aisada Uchugonova, Fraunhofer Institute of Biomedical Technology (Germany)
Sven Martin, JenLab GmbH (Germany)
Rainer Bückle, JenLab GmbH (Germany)
Karsten König, Fraunhofer Institute of Biomedical Technology (Germany)


Published in SPIE Proceedings Vol. 6442:
Multiphoton Microscopy in the Biomedical Sciences VII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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