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Proceedings Paper

In-vivo multiphoton-endoscopy of endogenous skin fluorophores
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Paper Abstract

Multiphoton tomography offers a painless method to examine patients under natural physiological conditions in vivo. Multiphoton excitation induces a weak autofluorescence of naturally endogenous fluorescent bio-molecules, such as flavines, NAD(P)H, metal-free porphyrines, components of lipofuscin, elastin and keratin. Additionally, collagen can be detected by second harmonic generation (SHG). Due to the nonlinearity, the effects occur only in a very tight focus, where the photon density is high enough. This leads to high axial and lateral resolution of <1μm without any need of a confocal detection and avoids out-of-focus damage. The limited depth range, given by the working distance of the focusing optics, is overcome with a gradient index-lens (GRIN-lens) based endoscope. In this work we present the first results of clinical applications in vivo of gradient-index lens endoscopes. Images of e.g. elastin and collagen (SHG) in the dermal layer of human skin are presented.

Paper Details

Date Published: 10 February 2007
PDF: 7 pages
Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421Y (10 February 2007); doi: 10.1117/12.702394
Show Author Affiliations
Alexander Ehlers, Fraunhofer-Institut for Biomedical Technology (Germany)
Saarland Univ. (Germany)
Selma Schenkl, Fraunhofer-Institut for Biomedical Technology (Germany)
Saarland Univ. (Germany)
Iris Riemann, Fraunhofer-Institut for Biomedical Technology (Germany)
Bernhard Messerschmidt, GrinTech GmbH (Germany)
Martin Kaatz, Friedrich Schiller Univ. (Germany)
Rainer Bückle, JenLab GmbH (Germany)
Karsten König, Fraunhofer-Institut for Biomedical Technology (Germany)
Saarland Univ. (Germany)
JenLab GmbH (Germany)


Published in SPIE Proceedings Vol. 6442:
Multiphoton Microscopy in the Biomedical Sciences VII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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