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Proceedings Paper

Imaging living cells with a combined high-resolution multi-photon-acoustic microscope
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Paper Abstract

With increasing demand for in-vivo observation of living cells, microscope techniques that do not need staining become more and more important. In this talk we present a combined multi-photon-acoustic microscope with the possibility to measure synchronously properties addressed by ultrasound and two-photon fluorescence. Ultrasound probes the local mechanical properties of a cell, while the high resolution image of the two-photon fluorescence delivers insight in cell morphology and activity. In the acoustic part of the microscope an ultrasound wave, with a frequency of GHz, is focused by an acoustic sapphire lens and detected by a piezo electric transducer assembled to the lens. The achieved lateral resolution is in the range of 1&mgr;m. Contrast in the images arises mainly from the local absorption of sound in the cells, related to properties, such as mass density, stiffness and viscose damping. Additionally acoustic microscopy can access the cell shape and the state of the cell membrane as it is a intrinsic volume scanning technique.The optical part bases on the emission of fluorescent biomolecules naturally present in cells (e.g. NAD(P)H, protophorphyrin IX, lipofuscin, melanin). The nonlinear effect of two-photon absorption provides a high lateral and axial resolution without the need of confocal detection. In addition, in the near-IR cell damages are drastically reduced in comparison to direct excitation in the visible or UV. Both methods can be considered as minimal invasive, as they relay on intrinsic contrast mechanisms and dispense with the need of staining. First results on living cells are presented and discussed.

Paper Details

Date Published: 13 February 2007
PDF: 7 pages
Proc. SPIE 6437, Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 64372A (13 February 2007); doi: 10.1117/12.702391
Show Author Affiliations
Selma Schenkl, Saarland Univ. (Germany)
Fraunhofer Institute of Biomedical Technology (Germany)
Eike Weiss, Fraunhofer Institute of Biomedical Technology (Germany)
Martin Stark, Fraunhofer Institute of Biomedical Technology (Germany)
Frank Stracke, Fraunhofer Institute of Biomedical Technology (Germany)
Iris Riemann, Fraunhofer Institute of Biomedical Technology (Germany)
Robert Lemor, Fraunhofer Institute of Biomedical Technology (Germany)
Karsten König, Saarland Univ. (Germany)
Fraunhofer Institute of Biomedical Technology (Germany)


Published in SPIE Proceedings Vol. 6437:
Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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